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¹ Guangxi Engineering Research Center of Processing and Storage of Characteristic and Advantage Aquatic Products, Guangxi Academy of Fishery Science, Nanning 530021, Guangxi, China
² Aquaculture Centre, Guangxi Aquatic and Animal Husbandry School, Nanning 530022, Guangxi, China
³ The Food Engineering and Technology Center, Guangxi Xiaoyanren Biotechnology Co., Ltd, Nanning 530017, Guangxi, China DOI : 10.4194/GA704 Viewed : 624 - Downloaded : 293 This study used Aeromonas hydrophila infection and culture of tilapia (Oreochromis mossambicus) renal cells to analyze the time dependent expression of miR-23a in tilapia renal cells infected with A. hydrophila. The current results showed that the expression of miR-23a changed significantly at different time points of infection. Using RNAhybrid software to predict the target site of miR-23a and construct a plasmid, the binding ability of the target gene 3`-UTR to miR-23a was verified through dual luciferase reporter gene assay. We found that target genes were reverse regulated, and tbk1, glut1 were identified as miR-23a target genes. Expression of ldha, ldhba, pdha 1a and pdha 1b was suppressed after overexpression of miR-23a. These results indicated that miR-23a was involved in the regulation of the immune response in tilapia kidney cells after A. hydrophila infection. tbk1 and glut1 are the target genes of miR-23a that can affect the expression of downstream genes by targeting GLUT1. The above results provide important insights into the role of miR-23a in regulating the immune response in bony fish and further understanding of miRNA-mediated multiple target genes to regulate innate immunity in fish. Keywords : miR-23a Oreochromis mossambicus Kidney cells Aeromonas hydrophila Immune response